Comparison of analytical methods for detection of perchloroethylene glutathione conjugates and application in liver fractions
Perchloroethylene (PER) is a degreasing solvent widely
used to replace the toxic trichloroethylene. However, PER
can also lead to nephrotoxicity via bioactivation by the
glutathione (GSH) conjugative pathway. In this study, a
postcolumn o-phtaldialdehyde (OPA)/N-acetylcysteine
(NAC) derivatisation method has been developed and
compared to existing methods for analysis of PER GSH
conjugates. Subsequently, the rate of this conjugation has
been studied for the first time in human subcellular
fractions. The specific activity of PER GSH conjugation in
humans shows significant interindividual differences and
is 10-fold lower compared to rats, indicating that humans
are less susceptible to nephrotoxicity via this pathway.
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